Masaki Mizutani, Isil Tulum, Yoshiaki Kinosita, Takayuki Nishizaka, Makoto Miyata,
Detailed Analyses of Stall Force Generation in Mycoplasma mobile Gliding,
Biophysical Journal 114, 1411-1419 (2018).
業績リスト
A03公募（2016-17） 西坂 崇之
論文等 | 原著論文
2018
Yoshiaki Kinosita, Yoshitomo Kikuchi, Nagisa Mikami, Daisuke Nakane, Takayuki Nishizaka,
Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body,
The ISME Journal 12, 838-848 (2018).
2017
Daisuke Nakane and Takayuki Nishizaka*,
Asymmetric distribution of type IV pili triggered by directional light in unicellular cyanobacteria,
Proceedings of the National Academy of Sciences 114, 6593-6598 (2017).
概要： 「バクテリア」は、もっとも単純な生命体の1つであり、大きさは1ミリの1000分の1程しかありません。ところが、動く仕組みに注目すると、実に多様で複雑な様式を発達させていることが知られています。本研究では、光合成微生物のラン藻が、細い糸のような繊維状構造物を持ち、その伸長収縮を繰り返す様子を撮影することに成功しました。さらに、独自の光学顕微鏡を駆使することで、この生命体は、指向性のある光という外部シグナルによって、その運動を制御する能力を持つことを明らかにすることができました。これは「視覚を備えたバクテリア」という点でも画期的です。光を感知するだけでなく、光の向きを認識するという事実は、単純な生命体が、高度な情報処理を達成していることをあらわしています。スパイダーマンのようなバクテリアは、非常に単純ではあるが初歩的な視覚を備えることで、自身の持つ「糸」を伸縮させる方向を自由自在に制御していると考えられます。
Shoko Fujimura, Yuko Ito, Mitsunori Ikeguchi, Kengo Adachi, Junichiro Yajima, Takayuki Nishizaka,
Dissection of the angle of single fluorophore attached to the nucleotide in corkscrewing microtubules,
Biochemical and Biophysical Research Communications 485, 614-620 (2017).
2016
Yoshiaki Kinosita, *Nariya Uchida, Daisuke Nakane, and *Takayuki Nishizaka,
Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum,
Nature Microbiology 1, 16148/1-9 (2016).
Akihiro Tanaka, Daisuke Nakane, Masaki Mizutani, Takayuki Nishizaka, *Makoto Miyata,
Directed binding of gliding bacterium, Mycoplasma mobile, shown by detachment force and bond lifetime,
mBio 7, e00455-16 (2016).
*Mitsuhiro Sugawa, Kei-ichi Okazaki, Masaru Kobayashi, Takashi Matsui, Gerhard Hummer, Tomoko Masaike, and *Takayuki Nishizaka,
F1-ATPase conformational cycle from simultaneous single-molecule FRET and rotation measurements,
Proceedings of the National Academy of Sciences of the United States of America 113, E2916-2924 (2016).
国際会議発表
2016
Invited
*Takayuki Nishizaka, Yoshiaki Kinosita, Takanobu. A. Katoh, Daisuke Nakane,
Structural and functional dynamics of nanoscale molecular motors,
The 31st International Congress on High-Speed Imaging and Photonics (Nov. 7-10, 2016), Osaka, Japan.
*Takayuki Nishizaka,
Visualization of dynamics of motors in archaella and type IV pili system,
Current and Future Perspectives in Active Matter (Oct. 28-29, 2016), Tokyo, Japan.
Poster
Kuzuu, S., Daisuke Nakane, *Y. Fukumori, A. Taoka, Takayuki Nishizaka,
Polarity of a bacterial nano-compass reversed by magnetic tweezers,
Magnetotactic Bacteria 5th International Meeting (Sep. 11-15, 2016), Provence, France.
*Kawamura, S., Y. Takaoka, A. Taoka, Daisuke Nakane, Takayuki Nishizaka, Y. Fukumori,
Visualization of flagellar rotation during magnetotactic motility using Magnetospirillum magneticum AMB-1,
Magnetotactic Bacteria 5th International Meeting (Sep. 11-15, 2016), Provence, France.
*Masaki Mizutani, Isil Tulum, Yoshiaki Kinosita, Takayuki Nishizaka, Makoto Miyata,
Force generated by single unit in Mycoplasma mobile gliding,
21st Congress of the International Organization for Mycoplasmology (Jul. 3-7, 2016), Brisbane, Australia.
*Masaki Mizutani, Isil Tulum, Yoshiaki Kinosita, Takayuki Nishizaka, Makoto Miyata,
Force generation based on novel energy conversion system in Mycoplasma mobile measured by laser trap,
7th OCARINA International Symposium in conjunction with Symposium in honor of Distinguished Professor Michael Nobel (Mar. 17-18, 2016), Osaka, Japan.
*Nagisa Mikami, Yuko Ito, Mitsuhiro Sugawa, Mitsunori Ikeguchi, Takayuki Nishizaka,
Conformational change of ab-interface in F1-ATPase detected by anisotropy of fluorescent nucleotide attached to catalytic sit,
Gordon Research Conference (Mar. 3-9, 2016), Hong Kong, China.
Nagisa Mikami, Yuko Ito, K. Adachi, Mitsunori Ikeguchi, *Takayuki Nishizaka,
Conformational Change in γ120 and γ240 of F1-ATPase from the Aspect of Difference of Nucleotides by Advanced TIRF Microscope,
Biophyscial Society 60th Annual Meeting (Feb. 27- Mar. 2, 2016), Los Angeles, USA.
Oral (contributed)
Yoshiaki Kinosita, Daisuke Nakane, Nariya Uchida, Makoto Miyata, *Takayuki Nishizaka,
Unitary Steps of Supermolecular Motility Machineries in Gliding Bacteria and Swimming Arcaea,
Biophyscial Society 60th Annual Meeting (Feb. 27- Mar. 2, 2016), Los Angeles, USA.
Poster
Takanobu A. Katoh, Koji Ikegami, Nariya Uchida, Toshihito Iwase, Tomoko Masaike, Mitsutoshi Setou, *Takayuki Nishizaka,
Separating Ciliary Beating Force into Dynein-Driven Force and Axoneme-Intrinsic Bending Force By 3-D Tracking Microscopy,
Biophyscial Society 60th Annual Meeting (Feb. 27- Mar. 2, 2016), Los Angeles, USA.
著書
2017
「Handbook of Photonics for Biomedical Engineering」Aaron Ho-Pui HoDonghyun KimMichael G. Somekh（編）
“3-D Single Particle Tracking Using Dual Images Divided by Prism: Method and Application to Optical Trapping” Takanobu A. Katoh, Shoko Fujimura, Takayuki Nishizaka
Springer（2017）, 755-766, ISBN : 978-94-007-5051-7
概要：本研究グループが独自に開発した3次元位置検出顕微鏡を光ピンセットに組み合わせ、新しい手法を開発。その詳細について技術的な視点から解説した。